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Acaricomes phytoseiuli : ウィキペディア英語版 | Acaricomes phytoseiuli
''Acaricomes phytoseiuli'' was discovered when a bacterium was isolated in the Netherlands from a diseased, surface-sterilized specimen of a female ''Phytoseiulus persimilis'' Athias-Henriot mite. ''Phytoseiulus persimilis'' is a predatory mite and ''Acaricomes phytoseiuli'', a pathogenic bacterium, causes a set of characteristic symptoms in the mite, which are termed "Non-Responding Syndrome" or "NR Syndrome". Dramatic changes in longevity, fecundity, and behavior are characteristic with this disease . The bacteria accumulate in the lumen of the mite's digestive tract and cause extreme degeneration of its epithelium. Infection with ''A. phytoseiuli'' greatly reduces the mite's attraction to herbivore-induced plant volatiles, and the mite is more prone to leave patches with ample prey.The disease is transmitted horizontally by means of feces and debris. The strain that was isolated was “CSC” (=DSM 14247T=CCUG 49701T). Differences between strain CSC compared to its closest phylogenetic neighbors are as follows: CSC utilizes glucose-1-phosphate and L-glutamic acid, and its colonies are more yellow in appearance as compared to its phylogenetic neighbors who are more cream/white in color.〔http://www.thelabrat.com/protocols/Bacterialspecies/Acaricomesphytoseiuli.shtml〕 PCR is a tool that can be used to help detect ''A. phytoseiuli'' in mites. The primers used are specific for ''A. phytoseiuli'' and are based on its 16S ribosomal DNA. ==Cell morphology== Using a phase-contrast microscope, cells appear as small, short rods, 0.5-0.8 x 1.0-1.5 micrometers in size. Optimal growth temperature for this organism is 25°C, with growth observed within the temperature range 15- 30 °C. To the naked eye, on a TSA plate, colonies appear smooth, circular, yellowish, and 1-2 mm in diameter. Growth can occur at pH values ranging from 6.0 to 9.5, with an optimal range of 6.0 to 8.0. ''Acaricomes phytoseiuli'' tests negative for the Oxidase reaction, but catalase activity is detectable. Trypticase soy agar(TSA) or trypticase soy broth (TSB) are the appropriate growth media for this organism. G+C content is 57.7%. ''Acaricomes phytoseiuli'' is a Gram-positive, aerobic, non-motile and non-spore-forming organism. *〔http://www.bacterio.cict.fr/bacdico/aa/acaricomes.html〕 Its peptidoglycan consists of the amino acids lysine, alanine, and glutamic acid in the respective ratios: 1.0:4.9:1.0. Cell wall sugars consist of galactose and glucose. The main fatty acids determined in this organism include 12-methyltetradecanoic acid and 14-methylhexadecanoic acid.〔http://genomesonline.org/cgi-bin/GOLD/bin/GOLDCards.cgi?goldstamp=Gi11061〕
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